How It Works

The BioSqueeze® Process

1. Fills cracks, fissures, and micro-annuli

BioSqueeze® is a proprietary fluid-based solution for forming calcium carbonate in micro-channels, permanently sealing them. The micron-size bacteria travel in the aqueous phase; they go where the water goes, including the smallest micro-channels and pore spaces. Advantages to calcium carbonate formation include:

  • Engineered permeability reduction – low viscosity fluids are injected downhole to fill voids with controlled deposition of crystalline calcium carbonate.
  • Bio-cementation – crystallized calcium carbonate bonds materials (e.g. sand, cement, steel) together.

2. Forms crystalline calcium carbonate

Once the solution occupies the space, BioSqueeze® forms crystalline calcium carbonate.  As mineralization continues to occur over several hours, the process enables permanent crystal growth in the cracks, fissures, and micro-annuli, resulting in a 100% inorganic natural seal.  There are many advantages over other sealing technologies, such as micro cements, resins, and liquid metals. Even micro cements are typically unable to penetrate the small fractures (less than 100 um) that are allowing gas migration behind the well casing. Resins liquid metals are expensive and difficult to place properly. No other wellbore sealing solution can do what BioSqueeze® does.

Photo Courtesy of Adrienne Phillips


BioSqueeze® is a fluid-based solution for forming calcium carbonate in micro-channels.


Why BioSqueeze® Is a Safe Process

Environmentally Friendly Technology

Biomineralization is an environmentally friendly technology that can be used to fill void spaces in the subsurface, such as micro-fractures in cement and porosity between sand particles. Biomineralization is much safer and more effective than other sealing technologies, such as high pH cements, toxic resins and metals. Crystalline minerals readily attach to rock, cements, and steel as shown in the following scanning electron microscopy images.

Using Naturally Occurring Soil Bacteria

Our process uses common, non-pathogenic, naturally occurring soil bacteria that have an aerobic metabolism (non-souring). The bacteria cease functioning when available oxygen is consumed or when the surrounding temperature exceeds 110-120°F. However, crystal formation persists for a while after the organisms become inactive. Also, biocide treatment can be resumed following a BioSqueeze® since it does not affect the minerals formed.

Mineralizing solutions used to form the calcium carbonate are common, widely available, safe chemicals. These are used as food additives, fertilizers, and agricultural feed products.

Photo Courtesy of Adrienne Phillips

Photo Courtesy of Adrienne Phillips

The silver bullet for the Oil and Gas industry

Solutions for all stakeholders

We work with small, mid-sized, and large operators in several basins throughout North America.
Whether you have one well, or one hundred, we can design solutions that work for all stakeholders.